| 產品編號 | bsm-63191R |
| 英文名稱 | eIF4EBP1 Recombinant Rabbit mAb |
| 中文名稱 | eIF4E結合蛋白重組兔單抗 |
| 別 名 | 4E-BP1; 4EBP1; BP-1; MGC4316; PHAS-I. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 2H10 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13 kDa |
| 檢測分子量 | 18 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human 4E BP1: 1-46 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. SWISS: Q13541 Gene ID: 1978 4E-BP1是一種分子量為10-12kDa 的酸性蛋白，與eIF4G 競爭結合。4E-BPs 對 eIF4E 的結合是可逆的，并依賴于 4E-BP 的磷酸化狀態。非磷酸化的4E-BP1 能與 eiF4E 牢固結合，而磷酸化的4E-BP1則不能。 而一些蛋白激酶Akt、 TOR、 MAPK、S6激酶和Cdc2是已知的能通過對35、45、69位的蘇氨酸殘基或64位的絲氨酸殘基進行磷酸化而使4E-BP1喪失對eIF4E結合能力的激酶。然而，并非所有的磷酸化事件都能 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with eIF4EBP1 monoclonal antibody, unconjugated (bsm-63191R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4EBP1 Monoclonal Antibody, Unconjugated (bsm-63191R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4EBP1 Monoclonal Antibody, Unconjugated (bsm-63191R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4EBP1 Monoclonal Antibody, Unconjugated (bsm-63191R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4EBP1 Monoclonal Antibody, Unconjugated (bsm-63191R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4EBP1 Monoclonal Antibody, Unconjugated (bsm-63191R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
