| 產品編號 | bsm-63179R |
| 英文名稱 | 14-3-3 alpha/beta/zeta/delta Recombinant Rabbit mAb |
| 中文名稱 | 14-3-3 α/β/ζ/δ重組兔單抗 |
| 別 名 | 14 3 3 protein beta; 14 3 3 protein beta/alpha; 14 3 3 protein zeta; 14 3 3 zeta; GW128; HS1; KCIP 1; N-terminally processed; YWHAB; YWHAD; YWHAZ. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4H5 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | 28 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human 14-3-3 alpha/beta: 1-50/246 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. SWISS: P31946 Gene ID: 7529 信號傳導（Signaling Intermediates）14-3-3蛋白是一個涉及調節細胞凋亡、促細胞分裂信號傳導和細胞周期關卡的蛋白質家族。它被認為是通過與絲氨酸殘基磷酸化的蛋白質的結合介導的信號傳導中的關鍵調節物。通過與Bad(相關死亡因子)的結合， 14-3-3 蛋白由于將Bad隔離于胞液而防止了細胞凋亡。 蛋白是14-3-3家族成員。它廣泛分布于哺乳動物、兩棲類、昆蟲、植物和酵母菌的真核生物高度保守性多功能蛋白質。 目前已知至少有16個成員。此抗體識別分子量為30-31kDa的14-3-3蛋白 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with 14-3-3 alpha/beta/zeta/delta monoclonal antibody, unconjugated (bsm-63179R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 14-3-3 alpha/beta/zeta/delta Monoclonal Antibody, Unconjugated (bsm-63179R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
