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phospho-IKB epsilon (Ser22) Rabbit pAb (bs-15582R)  
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產品編號 bs-15582R
英文名稱 phospho-IKB epsilon (Ser22) Rabbit pAb
中文名稱 NF-kappa-B抑制子epsilon抗體
別    名 IKB epsilon(phospho S22); p-IKB epsilon(phospho S22); I kappa B epsilon; I-kappa-B-epsilon; IkappaBepsilon; IkB E; IkB-E; IkB-epsilon; IKBE; IKBE_MOUSE; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFka  
研究領域 細胞生物  免疫學  染色質和核信號  信號轉導  轉錄調節因子  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,Rat (predicted: Human,Sheep,Cow)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 39 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human IKB epsilon around the phosphorylation site of Ser22: LR(p-S)LR 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Function:
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Subunit:
Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Serine phosphorylated; followed by proteasome-dependent degradation.

Similarity:
Belongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.

SWISS:
O00221

Gene ID:
4794

Database links:

Entrez Gene: 4794 Human

Entrez Gene: 18037 Mouse

Omim: 604548 Human

SwissProt: O00221 Human

SwissProt: O54910 Mouse

Unigene: 458276 Human

Unigene: 57043 Mouse



產品圖片
Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-phospho-IKB epsilon (Ser22) (bs-15582R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IKB epsilon (Ser22)) Polyclonal Antibody, Unconjugated (bs-15582R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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