| 產品編號 | bs-12286R |
| 英文名稱 | DDX3Y Rabbit pAb |
| 中文名稱 | 精子發育相關蛋白DDX3Y抗體 |
| 別 名 | DBY; DEAD(Asp-Glu-Ala-Asp) box polypeptide 3 Y linked; DEAD box protein 3 Y chromosomal; DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide Y chromosome; DDX3Y_HUMAN. |
| 研究領域 | 細胞生物 發育生物學 干細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 73 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human DDX3Y: 401-500/660 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections. Function: DEAD box proteins are putative RNA helicases, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as ribosome and spliceosome assembly, translation initiation and nuclear and mitochondrial splicing. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it has a homolog on the X chromosome. Mutations in DDX3Y cause male infertility, Sertoli cell-only syndrome or severe hypospermatogenesis, suggesting that it plays a key role in the spermatogenic process. Alternatively spliced variants, encoding the same protein, have been identified. Subunit: May interact with TDRD3. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Testis-specific. Expressed predominantly in spermatogonia. DISEASE: Note=DDX3Y is located in the 'azoospermia factor a' (AZFa) region on chromosome Y which is deleted in Sertoli cell-only syndrome. This is an infertility disorder in which no germ cells are visible in seminiferous tubules leading to azoospermia. Similarity: Belongs to the DEAD box helicase family. DDX3/DED1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: O15523 Gene ID: 8653 Database links: Entrez Gene: 8653 Human Entrez Gene: 26900 Mouse Omim: 400010 Human SwissProt: O15523 Human SwissProt: Q62095 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
