| 產品編號 | bs-11678R |
| 英文名稱 | NUP107 Rabbit pAb |
| 中文名稱 | 核孔復合體蛋白抗體 |
| 別 名 | 107 kDa nucleoporin; NPC proteins; NU107_HUMAN; Nuclear pore complex protein Nup107; Nucleoporin 107kDa; Nucleoporin Nup107; NUP 107; NUP 84; NUP84. |
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Specific References (1) | bs-11678R has been referenced in 1 publications.
[IF=5.182] Wei Lv. et al. Demonstration of intracellular trafficking, cytosolic bioavailability, and target manipulation of an antibody delivery platform. Nanomed-Nanotechnol. 2021 Feb;32:102315 IF ; Human.
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| 研究領域 | 染色質和核信號 神經生物學 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=3μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 106 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nuclear Pore Complex Proteins: 501-600/925 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is an essential component of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008] Function: Essential component of nuclear pore complex. Required for the assembly of peripheral proteins into the nuclear pore complex. Subunit: Forms part of the Nup160 subcomplex in the nuclear pore which is composed of Nup160, Nup133, Nup107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and Nup153 to the nucleus. Subcellular Location: Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Note=Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the nucleoporin Nup84/Nup107 family. SWISS: P57740 Gene ID: 57122 Database links: Entrez Gene: 57122 Human Entrez Gene: 103468 Mouse Omim: 607617 Human SwissProt: P57740 Human SwissProt: Q3TT06 Mouse SwissProt: Q3V395 Mouse SwissProt: Q569X8 Mouse SwissProt: Q8BH74 Mouse Unigene: 524574 Human Unigene: 12568 Mouse Unigene: 11398 Rat |
| 產品圖片 |
Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- Nuclear Pore Complex Proteins'Nup107 (bs-11678R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106kD
Observed band size: 106kD
Blank control(blue): 293T(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Nuclear Pore Complex Proteins/Nup107/AF488 Conjugated antibody (bs-11678R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
