| 產品編號 | bs-11492R |
| 英文名稱 | UNC5B Rabbit pAb |
| 中文名稱 | 神經突起誘導因子受體UNC5B抗體 |
| 別 名 | UNC5H2; Netrin receptor UNC5B; Netrin receptor UNC5B Precursor; p53 regulated receptor for death and life; p53 regulated receptor for death and life protein 1; p53-regulated receptor for death and life protein 1; p53RDL1; Protein unc-5 homolog 2; Protein |
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Specific References (4) | bs-11492R has been referenced in 4 publications.
[IF=5.16] Ding S et al. Macrophage-derived Netrin-1 participates in endometriosis-associated pain. Neurobiology of Disease.2019. IHC-F ; Human.
[IF=4.831] Shijie Wu. et al. High expression of UNC5B enhances tumor proliferation, increases metastasis, and worsens prognosis in breast cancer. Aging-Us. 2020 Sep 15; 12(17): 17079–17098 WB ; Human.
[IF=3.297] Shaojie Ding. et al. Macrophage-derived netrin-1 contributes to endometriosis-associated pain. Ann Transl Med. 2021 Jan; 9(1): 29 IHC ; Human.
[IF=1.49] Ko CL et al. Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. High Alt Med Biol. 2019 Jul 22. IHF ; Mouse.
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| 研究領域 | 心血管 細胞生物 神經生物學 血管內皮細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 101 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human UNC5B: 188-260/945 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the netrin family of receptors. This particular protein mediates the repulsive effect of netrin-1 and is a vascular netrin receptor. This encoded protein is also in a group of proteins called dependence receptors (DpRs) which are involved in pro- and anti-apoptotic processes. Many DpRs are involved in embryogenesis and in cancer progression. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011] Function: FunctionReceptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity. Subunit: Interacts with the cytoplasmic part of DCC (By similarity). Interacts with GNAI2 via its cytoplasmic part. Interacts (via death domain) with DAPK1 (via death domain). Subcellular Location: Membrane; Single-pass type I membrane protein. Note=Associated with lipid raft. Tissue Specificity: Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues. Post-translational modifications: Phosphorylated on cytoplasmic tyrosine residues (By similarity). Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis. Palmitoylation target the protein to the lipid rafts, and is required for pro-apoptotic activity. Similarity: Belongs to the unc-5 family. Contains 1 death domain. Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 2 TSP type-1 domains. Contains 1 ZU5 domain. SWISS: Q8IZJ1 Gene ID: 219699 Database links: Entrez Gene: 219699 Human Entrez Gene: 107449 Mouse Omim: 607870 Human SwissProt: Q8IZJ1 Human SwissProt: Q8K1S3 Mouse Unigene: 522997 Human Unigene: 290433 Mouse |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with UNC5B polyclonal antibody, unconjugated (bs-11492R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
