| 產品編號 | bs-6119R |
| 英文名稱 | ZNF185 Rabbit pAb |
| 中文名稱 | 鋅指蛋白185抗體 |
| 別 名 | LIM domain protein ZNF185; P1 A; Zinc finger protein 185(LIM domain); ZN185_HUMAN. |
| 研究領域 | 腫瘤 染色質和核信號 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Dog) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 76(human)/39(mo, kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF185: 551-650/689 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
ZNF185 encodes a LIM domain zinc finger protein. It may be involved in the regulation of cellular proliferation and/or differentiation. Function: May be involved in the regulation of cellular proliferation and/or differentiation. Subcellular Location: Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Tissue Specificity: Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood. Similarity: Contains 1 LIM zinc-binding domain. SWISS: O15231 Gene ID: 7739 Database links: Entrez Gene: 7739 Human Entrez Gene: 22673 Mouse Omim: 300381 Human SwissProt: O15231 Human SwissProt: Q62394 Mouse Unigene: 16622 Human Unigene: 1161 Mouse |
| 產品圖片 |
Sample:
MCF-7(Human) Cell Lysate at 40 ug
A431(Human) Cell Lysate at 40 ug
U2os(Human) Cell Lysate at 40 ug
Primary: Anti-ZNF185 (bs-6119R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76/39 kD
Observed band size: 67 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (bs-6199R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
