| 產(chǎn)品編號 | bs-4042R |
| 英文名稱 | Triosephosphate isomerase Rabbit pAb |
| 中文名稱 | 磷酸丙糖異構(gòu)酶抗體 |
| 別 名 | TIM; TPI 1; TPI; TPI1; Triose phosphate isomerase 1; Triose phosphate isomerase; Triosephosphate isomerase 1; Triosephosphate isomerase; MGC88108; TPIS_HUMAN. |
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Specific References (3) | bs-4042R has been referenced in 3 publications.
[IF=7.25] Yao, Chun, et al. "Role of FADD Phosphorylation in Regulating Glucose Homeostasis: from Proteomic Discovery to Physiological Validation." Molecular & Cellular Proteomics (2013). WB ; Mouse.
[IF=5.682] Jie Jiang. et al. Glycolysis- and immune-related novel prognostic biomarkers of Ewing's sarcoma: glucuronic acid epimerase and triosephosphate isomerase 1. Aging-Us. 2021 Jul 15; 13(13): 17516–17535 IHC ; Human.
[IF=3.752] Xuejiao An. et al. miR-1285-3p targets TPI1 to regulate the glycolysis metabolism signaling pathway of Tibetan sheep Sertoli cells. PLOS ONE. 2022 Sep;17(9):e0270364 WB ; Sheep.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Triosephosphate isomerase: 151-249/249 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq] Subunit: Homodimer. Post-translational modifications: The initiator methionine for isoform 2 is removed. DISEASE: Triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]: Autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the triosephosphate isomerase family. SWISS: P60174 Gene ID: 7167 Database links: UniProtKB/Swiss-Prot: P60174.3 Entrez Gene: 7167 Human Entrez Gene: 21991 Mouse Omim: 190450 Human SwissProt: P60174 Human ????磷酸丙糖異構(gòu)酶（Triose-phosphate isomerase，通常簡稱為TPI或TIM）是一種能夠催化丙糖磷酸異構(gòu)體在二羥丙酮磷酸和D型甘油醛-3-磷酸之間轉(zhuǎn)換。 ????磷酸丙糖異構(gòu)酶在糖酵解中具有重要作用，對于有效的能量生成是必不可少的。磷酸丙糖異構(gòu)酶被發(fā)現(xiàn)存在于幾乎所有的生物體，包括哺乳動物、昆蟲、真菌、植物和大多數(shù)細菌中。 ????如果人體缺乏磷酸丙糖異構(gòu)酶，會導致嚴重的神經(jīng)系統(tǒng)紊亂，該病癥被稱為磷酸丙糖異構(gòu)酶缺乏癥。 |
| 產(chǎn)品圖片 |
Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Triosephosphate isomerasee (bs-4042R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Triosephosphate isomerase (bs-4042R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated(bs-4042R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
