| 產品編號 | bs-3156R |
| 英文名稱 | phospho-GCN2 (Thr667) Rabbit pAb |
| 中文名稱 | 磷酸化蛋白激酶GCN2蛋白抗體 |
| 別 名 | GCN2(phospho T667); p-GCN2(phospho T667); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-l |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 181 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667: PG(p-T)PP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. Tissue Specificity: Widely expressed. Post-translational modifications: Autophosphorylated on threonine residues. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. SWISS: Q9P2K8 Gene ID: 440275 Database links: Entrez Gene: 440275 Human Entrez Gene: 27103 Mouse Omim: 609280 Human SwissProt: Q9P2K8 Human SwissProt: Q9QZ05 Mouse Unigene: 656673 Human Unigene: 217616 Mouse Unigene: 8333 Rat GCN2蛋白的作用；可能是將短期記憶變成長期記憶的一個主控調節因子， GCN2蛋白在長期記憶形成中功能的發現將可能幫助研究人員開發出提高記憶力喪失的病人提高記憶力。 |
| 產品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170/181 kD
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GCN2 (Thr667) (bs-3156R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 181 kD
Observed band size: 170 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (bs-3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
