| 產品編號 | bs-0129R |
| 英文名稱 | Nogo R Rabbit pAb |
| 中文名稱 | 軸索過度生長抑制因子受體/Nogo受體抗體 |
| 別 名 | NgR; Nogo-66 receptor; Nogor; NogoR; Nogo-R; Reticulon 4 receptor; Rtn4r; RTN4R_RAT. |
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Specific References (1) | bs-0129R has been referenced in 1 publications.
[IF=3.216] Jing Wanget al. Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling. Drug Des Devel Ther
. 2020 Jul 15;14:2775-2787. WB ; rat.
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| 研究領域 | 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Nogo R: 151-350/473 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Axons are essential for neuronal communication but they do not regenerate after injury to the adult mammalian brain or spinal cord. Failed regeneration is due in part to the production of a potent axonal growth inhibitor, Nogo, by myelinating cells. The finding of a high affinity axonal receptor for the extracellular domain of Nogo provides the first insight into the basis of Nogo action. Disrupting the interaction of Nogo with the Nogo-66 receptor may facilitate axonal regeneration in vivo. The protein is dubbed the Nogo receptor because it binds with several other proteins that block neural growth. It is found to be ubiquitous in the brain and spinal cord. Function: Receptor for RTN4, OMG and MAG. Mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. Acts in conjunction with RTN4 and LIGO1 in regulating neuronal precursor cell motility during cortical development (By similarity). Subunit: Homomultimer. Interacts with LINGO1. Interacts with KIAA0319L. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Similarity: Belongs to the Nogo receptor family. Contains 8 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. SWISS: Q99PI8 Gene ID: 65079 Database links: Entrez Gene: 65078 Human Entrez Gene: 65079 Mouse Omim: 605566 Human SwissProt: Q9BZR6 Human SwissProt: Q99PI8 Mouse Unigene: 30868 Human Unigene: 40149 Mouse |
| 產品圖片 |
Sample:
Hippocampus (Rat) Lysate at 40 ug
Primary: Anti- Nogo R (bs-0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Brain (Rat) Lysate at 40 ug
Primary: Anti- Nogo R (bs-0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- Nogo R (bs-0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Nogo R (bs-0129R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nogo-R Polyclonal Antibody, Unconjugated(bs-0129R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
