| 產品編號 | bs-0519R |
| 英文名稱 | ATF3 Rabbit pAb |
| 中文名稱 | 活化轉錄因子3抗體 |
| 別 名 | Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription factor ATF-3; TF3_HUMAN; cAMP-dependent transcrip |
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Specific References (10) | bs-0519R has been referenced in 10 publications.
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 細胞凋亡 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow,Chicken,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ATF3: 51-150/181 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]. Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Subunit: Binds DNA as a homodimer or a heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. SWISS: P18847 Gene ID: 467 Database links: Entrez Gene: 467 Human Entrez Gene: 11910 Mouse Omim: 603148 Human SwissProt: P18847 Human SwissProt: Q60765 Mouse Unigene: 460 Human Unigene: 2706 Mouse Unigene: 9664 Rat 轉錄調節因子（Transcriptin Regulators） 活化轉錄因子-3(屬于activation transcription factor/cAMP responsive element binding protein家族)是真核細胞轉錄因子，同屬于ATF/CREB家族。參與由cAMP或某些病毒蛋白質所誘導基因轉錄的調節。 轉錄激活因子3(activating transcription factor 3,ATF3)屬含亮氨酸拉鏈結構的轉錄因子ATF/CREB家族成員，分子量20kDa,在大部分正常細胞中呈低濃度穩態表達。在正常組織中含量很低，在應激狀態是表達增加，形成二聚體。ATF3是個應激早期快反應基因，體內外可被一系列損傷性應激信號快速誘導表達。ATF3不僅在應激反應中是個關鍵性的調控因子，還廣泛參與了機體穩態維持、創傷愈合、細胞黏附、腫瘤形成、凋亡以及信號傳導等生理和病理過程。ATF3與細胞生存和凋亡有關，但在不同的細胞狀態下發揮不同甚至相反的作用。 |
| 產品圖片 |
Sample:
Lane 1: Human 293T cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human A549 cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-ATF3 (bs-0519R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 20 kDa
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(bs-0519R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
